Primer3 0.4.0 ^new^ Info

Primer3 0.4.0 is an early release of Primer3, a widely used open-source program for designing PCR primers and oligonucleotides. This version contains core features for automated primer design including basic melting temperature (Tm) calculations, product size constraints, and primer-pair scoring.

hosted by the University of Tartu. It remains a reliable, "no-frills" tool for quickly generating high-quality oligonucleotides for everything from standard PCR to primer3 0.4.0

| Feature | Primer3 1.1.4 | Primer3 2.3.6 | | |---------|---------------|---------------|-------------------| | Tm model | Breslauer 86 | SantaLucia 98 | SantaLucia 98 | | Salt correction | 1M only | Owczarzy 04 | Owczarzy 04 | | Degenerate primers | No | Yes | Yes | | Multiplex penalty | No | Yes | Partial (output only) | | API stability | Poor | Good | Excellent | | Thread safety | No | No | Yes | | Memory footprint | Low | Medium | Very low | Primer3 0